Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: M 6 A -mediated lncRNA SCIRT stability promotes NSCLC progression through binding to SFPQ and activating the PI3K/Akt pathway

doi: 10.1007/s00018-025-05594-z

Figure Lengend Snippet: SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated lncRNA-related mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001

Article Snippet: Arraystar m 6 A lncRNA epitranscriptomic microarrays were used to screen differentially m 6 A-methylated lncRNAs in NSCLC, and array figures were shown in Supplementary Fig. . A total of 39 differential metylated candidates were identified with 24 upregulated genes and 15 downregulated genes (Table ).

Techniques: Modification, Expressing, Methylation

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Long noncoding RNA AK144717 exacerbates pathological cardiac hypertrophy through modulating the cellular distribution of HMGB1 and subsequent DNA damage response

doi: 10.1007/s00018-024-05464-0

Figure Lengend Snippet: Establishment of cardiac failure model and identification of altered lncRNAs. A–C . Echocardiography and quantitative analysis ( n = 6–7). D–E . Representative images of hematoxylin and eosin (H&E) staining (upper) and wheat germ agglutinin (WGA) staining ( n = 6–7). Scale bar: 100 μm. F . Quantitative analysis of the heart weight (HW)/body weight (BW) ratio ( n = 6–7). G . qPCR analysis of ANP and BNP mRNA expressions ( n = 6–7). H . Heat map of the top 20 altered lncRNAs in the heart. The lncRNAs with raw intensities over 20 in the microarray sequencing report are framed in red. I . qPCR analysis of lncRNAs in the samples applied for microarray sequencing ( n = 3). J . qPCR validation of LncRNA AK144717 and Gm4316 (ENSMUST00000212411) in all the mice of sham and TAC group ( n = 6–7). K. qPCR analysis of LncRNA AK144717 expression in the TAC models of different time ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS means not significant between groups

Article Snippet: The ventricular samples from 3 mice operated by TAC for 6 weeks and 3 mice with sham surgery were sent to Shanghai KangChen Bio-tech Company to detect the differentially expressed lncRNAs by using Arraystar mouse lncRNA microarray V4.

Techniques: Staining, Microarray, Sequencing, Biomarker Discovery, Expressing

Journal: bioRxiv

Article Title: The long non-coding RNA NEAT1 regulates the transcriptional landscape of cardiomyocytes

doi: 10.1101/2024.06.27.600932

Figure Lengend Snippet: (A) Experimental setup to detect the deregulated lncRNA in hearts subjected to HF, HF+AAV9-SERCA2a relative to Sham hearts (B) Microarray based profiling of RNA isolated from rat hearts subjected to Myocardial infarction and rescue by Serca2a gene therapy. (C) qRT-PCR of Neat1 from RNA isolated from rat ventricle after MI and MI+Serca2a gene therapy. (D) qRT-PCR for detecting the change in expression of ANP, β-MHC, Neat1, and Neat1.2 in primary neonatal rat ventricular myocytes (NRCM) challenged with 100 µM Isoproterenol for 48 h. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between both groups. (E) Neat1 expression levels detected by qRT-PCR and ejection fraction (EF%) throughout heart failure progression (TAC, transverse aortic constriction; n = 5–8). * p value < 0.05, t-tests were performed to compute statistical differences between Sham and TAC. (F) Neat1 expression levels and ejection fraction (EF%) throughout heart failure progression after subjecting mice to 15 mg/kg/day, 30mg/kg/day, and 50 mg/kg/day of isoproterenol for 10 days. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between saline and isoproterenol groups. (G) qRT-PCR for detecting the expression of total Neat1 and Neat1.2 in adult mice cardiomyocytes and adult mice non-myocyte fraction.

Article Snippet: Rat lncRNA microarray (4X44K, Arraystar) was performed in the heart samples isolated from the rats that were subjected to proximal coronary artery ligation (HF), HF+ SERCA2A gene therapy.

Techniques: Microarray, Isolation, Quantitative RT-PCR, Expressing, Saline

Journal: Aging (Albany NY)

Article Title: Calycosin stimulates the proliferation of endothelial cells, but not breast cancer cells, via a feedback loop involving RP11-65M17.3, BRIP1 and ERα

doi: 10.18632/aging.202641

Figure Lengend Snippet: The effects of calycosin treatment on lncRNA profiles in HUVECs.

Article Snippet: Labeled cDNA was subjected to hybridization using the Human lncRNA OneArray Plus microarray (Phalanx Biotech Group, Taiwan), followed by scanning using an Agilent scanner (Agilent Technologies, USA).

Techniques: